AFRICA CARDIOVASCULAR JOURNAL OF AFRICA • SAHS Biennial Congress 16-18 September 2022 62 Submission ID: 1383 Introduction Hypertension is a major risk factor for cardiovascular disease with the cause of essential hypertension remaining unknown. Rat models used to study hypertension include the spontaneously hypertensive rat (SHR) which was bred from the Wistar Kyoto (WKY) rats. Studies have shown an association between infection with the human ulcer causing bacterium Helicobacter pylori and hypertension, increased blood pressure (BP) and a decrease in BP following successful eradication. The aim of this study was to determine the impact of eradicating Helicobacter spp, in WKY and SHR rat models of hypertension. Methods Ethics approval for the study was obtained. SHR and WKY rats on normal chow and ad lib access to water were habituated to tail cuff measures. Rats were treated by gavage for 3 days with standard antibiotics to either eradicate Helicobacter spp (n=6 per strain) or other non-Helicobacter micro-organisms (n=6 per strain). Eradicated rats (negative stool PCR) were re-infected with the human pathogen H pylori. Results Antibiotic treatment resulted in BP decreasing from 175±6mmHg to 120±5mmHg (p=0.0039), and 139±3mm Hg to 113±6mm Hg (p=0.0065) in SHR and WKY rats respectively, without differences between eradicated WKY and SHR. BP increases after reinfection were modest and did not attain pre-eradication levels. Conclusion BP in SHR decreased to those of the ancestral WKY rat strain after treatment to eradicate Helicobacter spp. The modest BP increase following re-infection with H pylori may reflect the use of a non-rodent species. Further work is needed determine the role of Helicobacter spp. in the etiology of hypertension. Name: Presenting Author Information Article Category Abstract Title Surgery, Health Sciences, University of the Witwatersrand, Johannesburg geoffrey.candy@wits.ac.za English Abstract Basic Sciences Eradication of Helicobacter spp. in spontaneously hypertensive rats reduces blood pressure to that of ancestral Wistar Kyoto rats Author Affiliation: Email: Sarhana Dinat Authors Name & Surname Title Expertise Affiliation Email Country Sarhana Dinat* Ms MSc Surgery, University of the Wiwatersrand geoffrey.candy29@wits.ac.za South Africa Anza Thiba Ms MSc Surgery, University of the Wiwatersrand anzathiba@gmail.com South Africa Hannah Bint Ebraheem Ms MSc Surgery, University of the Wiwatersrand South Africa Ayodeji Idowu Dr PhD Surgery, University of the Wiwatersrand South Africa Karl Rumbold Prof PhD (Microbiology) Microbiology, University of the Witwatersrand karl.rumbold@wits.ac.za South Africa Geoffrey Candy Prof PhD (Biochemistry) Surgery, University of the Wiwatersrand geoffrey.candy@wits.ac.za South Africa POSTER PRESENTATION
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