AFRICA Cardiovascular Journal of Africa • SAHS Biennial Congress 2024 18 Submission ID: 1645 Introduction Chronic kidney disease (CKD) is a multifactorial progressive condition that has a strikingly adverse impact on cardiovascular disease risk. The identification of CKD specific effects on the cardiovascular system is compounded by common comorbidities such as hypertension, diabetes, and cardiovascular disease. This complexity necessitates the development of animal models that can determine the distinct pathophysiological mechanisms of CKD and its cardiovascular impacts. The present study was designed to delineate the distinct cardiovascular outcomes associated with CKD only versus CKD with concurrent hypertension. We aimed to induce CKD in Wistar Kyoto rats (WKYs), representing a model of lone CKD, and Spontaneously Hypertensive Rats (SHRs), representing a model of CKD with comorbid hypertension. Methods Both WKY and SHR were divided into control and adenine-treated subgroups. The adenine treated groups were given adenine supplemented diet for 8 weeks. Prior to terminations, animals were placed in metabolic cages for 8 hours to collect urine. On the day of terminations, under anaesthesia, a catheter was inserted into the carotid artery to measure central pressures and echocardiography was performed to assess cardiac function. Pulsepenlab was used to determine aortic function. Blood samples were collected via cardiac puncture. Thereafter, the heart and kidneys were harvested and weighed. Results The urea concentration was larger in SHR-treated rats compared to those in other groups (P= 0.028) Central systolic blood pressure (P<0.001), pulse pressure (P<0.02) and mean arterial pressure (P<0.001) were larger in SHR and SHR-treated groups compared to the WKY and WKY-treated groups. The SHR-treated group had a larger pulse pressure compared the WKY-treated group (P<0.001). Forward wave pressure (P<002), augmentation index (P=006) and augmented pressure (P=0.004) were increased in SHR-treated compared to WKY and WKY-treated groups. Left ventricular mass adjusted to body weight was higher in the SHR treated (P=0.02) compared to other groups. Right kidney mass adjusted for body weight was larger in the SHR-treated group than in the SHR (P = 0.0004) and WKY control groups (P = 0.002). The WKY-treated group also had heavier right sided kidneys than the WKY control group. Left kidney mass adjusted for body weight was larger in the SHR-treated group than in the WKY (P = 0.0002) and the SHR control groups (P = 0.006). Conclusion SHRs experience a greater susceptibility to adenine-induced kidney impairment than WKYs. Adenine-induced kidney impairment causes more impaired aortic function, left ventricular mass and kidney weight in SHRs than in WKYs. The current study design can allow for determining the differential impact of lone CKD versus CKD with comorbid hypertension on cardiovascular function and structure. Name: Presenting Author Information Article Category Abstract Title University of the Witwatersrand, Johannesburg South Africa oluwatosin.tade@wits.ac.za English Abstract Researchers/Clinicians - Early, mid & senior career Differential Responses to Adenine-Induced Chronic Kidney Disease in WKY and SHR Models Author Affiliation: Email: Grace Tade Science Theme Basic Authors Name & Surname Title Expertise Affiliation Email Country Oluwatosin Grace Tade Dr Early career Wits University oluwatosin.tade@wits.ac.za South Africa Makabongwe Mazibuko Ms Student Wits University 1353904@students.wits.ac.za South Africa Maud Dzikunu Ms Student Wits University 1813250@students.wits.ac.za South Africa Ursula Mariani Ms Student Wits University ursula.mariana@wits.ac.za South Africa Patience Mahema Ms Student Wits University 1432522@students.wits.ac.za South Africa Nonhlanhla Mthembu Dr Researcher Wits University nonhlanhla.mthembu@wits.ac.za South Africa Vernice Peterson Dr Researcher Wits University vernice.peterson@wits.ac.za South Africa Angela Woodiwiss Prof Senior Academic and researcher Wits University angela.woodiwiss@wits.ac.za South Africa Patrick Dessein Prof Clinician and researcher Wits University patrick.dessein22@gmail.com South Africa ORAL PRESENTATION
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