Cardiovascular Journal of Africa • SAHS Biennial Congress 2024 29 AFRICA Authors Name & Surname Title Expertise Affiliation Email Country Refenshe Amandu's Nthlane Miss Sleep and cardiovascular physiology Integrated Molecular Physiology Research Initiative, The Wits Health Consortium, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa 1448621@students.wits.ac.za South Africa Karine Scheuermaier Associate professor Sleep and pain physiology Wits Sleep Laboratory, Brain Function Research Group, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa karine.scheuermaier@wits.ac.za South Africa Siluleko Advice Mkhize Mr Cardiovascular physiology Integrated Molecular Physiology Research Initiative, The Wits Health Consortium, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa 843928@students.wits.ac.za South Africa Frédéric Michel Associate professor Cardiovascular physiology Integrated Molecular Physiology Research Initiative, The Wits Health Consortium, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa frederic.michel@wits.ac.za South Africa Table 3. 1: Characteristics of 5-month-old female ovariectomized and sham operated SHR after10-week chronic phase shift or control light protocol. Characteristics Sham Control light n=9 Sham CPS n=9 Ova Control light n=9 Ova CPS n=9 AUC OGTT, min*mmol/L Peak OGTT, mmol/L Final body mass, g Heart mass, g Left ventricle mass, g Liver mass, g Kidney mass, g 28.3 ± 4.2 7.2 ± 1.3 191 ± 10 0.86 ± 0.06 0.64 ± 0.04 7.1 ± 0.5 0.66 ± 0,07 29.6 ± 5.9 8.7 ± 2.2 193 ± 11 0.88 ± 0.06 0.65 ± 0.05 6.8 ± 0.4† 0.64 ± 0.09 28.0 ± 3.7 7.1 ± 1.5 251 ± 15*** 0.95 ± 0.07*** 0.72 ± 0.04*** 8.1 ± 0.8*** 0.71 ± 0.05* 27.6 ± 4.3 7.1 ± 1.9 250 ± 9*** 0.95 ± 0.06*** 0.71 ± 0.05*** 7.6 ± 0.5***† 0.69 ± 0.06* Normalized heart mass, g/100g Normalized left ventricle mass, g/100g Normalized liver mass, g/100g Normalized kidney mass, g/100g LDL, ug/mL 0.45 ± 0.03 0.34 ± 0.02 3.7 ± 0.3 0.34 ± 0.03 9.1 ± 6.5 0.46 ± 0.04 0.34 ± 0.02 3.5 ± 0.2† 0.33 ± 0.04 13.9 ± 10.0 0.38 ± 0.02*** 0.29 ± 0.01*** 3.2 ± 0.2*** 0.29 ± 0.02*** 9.7 ± 6.6 0.38 ± 0.02*** 0.29 ± 0.02*** 3.1 ± 0.2***† 0.28 ± 0.02*** 15.8 ± 12.1 Data expressed as mean ± SD. SHR, spontaneously hypertensive rats; CPS, chronic phase shift; Ova, ovariectomy; AUC, area under graph; OGTT, oral glucose tolerance test; LDL, low-density lipoprotein. *, p < 0.05 vs sham-operated groups; **, p < 0.01 vs sham-operated groups; ***, p < 0.001 vs sham-operated groups; †, p ≤ 0.05 vs control light groups. Table 3. 2: M–mode and M–mode derived echocardiographic parameters of 5-month-old female ovariectomized and sham operated SHR after 10-week chronic phase shift or control light protocol. Parameters Sham Control light n=9 Sham CPS n=9 Ova Control light n=9 Ova CPS n=9 Interventricular (mm) IVSTd IVSTs Left ventricular diameters (mm) LVEDd LVEDs Posterior wall thickness (mm) LVPWTd LVPWTs Derived Relative wall thickness FSend, % FSmid, % EF, % SV, µL 2.4 ± 0.3 3.4 ± 0.1 4.5 ± 0.6 2.4 ± 0.4 2.1 ± 0.6 2.8 ± 0.6 1.0 ± 0.4 48.2 ± 3.3 23.1 ± 3.3 79.6 ± 3.3 76.7 ± 23.2 2.3 ± 0.4 3.3 ± 0.5 4.6 ± 0.7 2.4 ± 0.5 2.1 ± 0.6 2.8 ± 0.5 1.0 ± 0.4 49.0 ± 5.2 23.1 ± 3.7 80.1 ± 4.8 79.9 ± 24.5 2.2 ± 0.5 3.1 ± 0.7 5.1 ± 0.5* 3.0 ± 0.7** 2.3 ± 0.4 2.9 ± 0.5 0.9 ± 0.2 41.7 ± 10.0** 20.4 ± 5.5 71.2 ± 12.5** 87.8 ± 19.2 2.7 ± 0.4 3.5 ± 0.3 5.0 ± 0.4* 3.0 ± 0.4** 2.0 ± 0.5 2.6 ± 0.6 0.8 ± 0.3 40.5 ± 6.0** 19.9 ± 4.7 70.6 ± 7.2** 84.9 ± 16.9 Data expressed as mean ± SD. SHR, spontaneously hypertensive rats; CPS, chronic phase shift; Ova, ovariectomy; IVSTd, intraventricular septal diameter end diastole; IVSTs, intraventricular septal diameter end systole; LVEDd, left ventricular end-diastolic diameter; LVEDs, left ventricular end-diastolic diameter; LVPWTd, left ventricular posterior wall thickness in diastole; LVPWTs, left ventricular posterior wall thickness in systole; FSend, endocardial fractional shortening; FSmid, mid wall fractional shortening; EF, ejection fraction; SV, stroke volume. *, p ≤ 0.05 vs sham-operated groups; **, p < 0.01 vs sham-operated groups Table 3. 3: Tissue and pulse Doppler echocardiography parameters of 5-month-old female ovariectomized and sham-operated SHR after 10-week chronic phase shift or control light protocol. Parameters Sham Control light n=9 Sham CPS n=9 Ova Control light n=9 Ova CPS n=9 Tissue doppler e’, cm/s 5.1 ± 0.8 6.4 ± 2.4 6.1 ± 0.7 5.6 ± 1.2 a’, cm/s s’, cm/s Pulse doppler E, cm/s A, cm/s Derived E/A E/e’ e’/a’ 3.3 ± 0.5 4.8 ± 0.7 84.3 ± 18.8 52.9 ± 20.6 1.71 ± 0.45 17.5 ± 7.1 1.6 ± 0.3 4.3 ± 0.9 5.3 ± 0.4 86.6 ± 26.7 57.5 ± 20.3 1.57 ± 0.35 15.7 ± 9.0 1.6 ± 0.7 3.7 ± 0.6 4.6 ± 0.7 86.3 ± 22.8 57.6 ± 20.3 1.62 ± 0.47 14.4 ± 3.9 1.7 ± 0.3 4.4 ± 1.9 4.6 ± 1.1 89.9 ± 10.4 55.9 ± 15.3 1.73 ± 0.51 17.0 ± 4.8 1.4 ± 0.5 Data expressed as mean ± SD. SHR, spontaneously hypertensive rats; CPS, chronic phase shift; Ova, ovariectomy; e’, early peak tissue lengthening velocity at the lateral mitral annulus; a’, late peak tissue lengthening velocity at the lateral mitral annulus; s’, peak systolic velocity; E, maximum early mitral inflow velocity; A, maximum late mitral inflow velocity; E/e’, early diastolic mitral inflow velocity to early diastolic mitral annulus velocity; E/A, early to late diastolic filling velocity ratio; e’/a’, early to late peak tissue lengthening velocity ratio. Submission ID: 1638 continued POSTER PRESENTATION
RkJQdWJsaXNoZXIy NDIzNzc=