Cardiovascular Journal of Africa • SAHS Biennial Congress 2024 5 AFRICA Submission ID: 1614 Introduction Hypertension drives the development of concentric left ventricular hypertrophy (LVH). However, the relative contribution of pentraxin-3 (PTX-3) in the hypertrophic response to pressure overload has not been adequately elucidated. Aim: We sought to investigate the role of PTX-3 in the development of LVH in spontaneously hypertensive rats (SHR), untreated and treated with either captopril (an ACE inhibitor) or hydralazine (a non-specific vasodilator). Methods Three-month-old SHR received either 20 mg/kg/day hydralazine (SHR+H, n=6), 40 mg/kg/day captopril (SHR+C, n=6), or plain gelatine cubes (untreated SHR, n=7) orally for 4 months. Wistar Kyoto rats (WKY, n=7) were used as the normotensive controls. Blood pressure (BP) was measured using the tail-cuff method. At termination, cardiac geometry and function under anaesthesia were determined using M-mode echocardiography. Following termination, the blood, and the left ventricles (LV) were sampled. Circulating levels of inflammatory markers were measured in plasma by ELISA and relative mRNA expression of PTX-3 was determined in the LV by RT-PCR. Results Untreated SHR exhibited greater systolic BP and relative wall thickness (RWT) compared to WKY. Captopril and hydralazine normalised BP but only captopril reversed hypertrophic changes in SHR. Circulating PTX-3 levels were elevated in untreated SHR but normalised with captopril and hydralazine. Circulating PTX-3 was positively associated with systolic BP but lacked independent relations with indices of LVH. In addition, LV relative mRNA expression of PTX-3 was similar between the groups. Conclusion PTX-3, a circulating marker of hypertension, may not be involved in the development of LVH in SHR. Name: Presenting Author Information Article Category Abstract Title Integrated Molecular Physiology Research Initiative, School of Physiology, Faculty of Health Sciences, University of the Witwatersrand siluleko.mkhize@wits.ac.za English Abstract Students - Currently enrolled postgraduate students Involvement of pentraxin-3 in the development of hypertension but not left ventricular hypertrophy in spontaneously hypertensive rats Author Affiliation: Email: Siluleko Mkhize Science Theme Basic Author Name & Surname Title Expertise Affiliation Email Country Siluleko Mkhize Mr Molecular & Cellular Molecular Physiology Research Initiative siluleko.mkhize@wits.ac.za South Africa ORAL PRESENTATION
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